Journal: Frontiers in Oncology

Article Title: Multi-Faceted Effects of ST6Gal1 Expression on Precursor B-Lineage Acute Lymphoblastic Leukemia

doi: 10.3389/fonc.2022.828041

Figure Lengend Snippet: BCP-ALL cells contain high levels of α2-6 sialylation. (A) Western blot of different BCP-ALL cell lines probed with SNA lectin to specifically detect α2-6-linked sialic acids on glycoproteins. GAPDH, loading control. Location of molecular weight standards to the right. (B) Analysis of N-glycans in RS4;11 cells as previously described . Combined results of 15 individual RS4;11 cell samples. Overall, more than 65% of all identified N-glycans were found to be sialylated with 7.4% in α2-3, 14.1% in α2-3/6, and 45.1% in α2-6 attachment.

Article Snippet: BCP-ALL cells were lysed in Triton T-100 lysis buffer with glycerol at pH 7.4 (Alfa Aesar, Cat#J63866AK) and glycoproteins were captured with SNA-biotin (Vector labs Cat #B-1305).

Techniques: Western Blot, Molecular Weight

Journal: Frontiers in Molecular Neuroscience

Article Title: Physiological Concentrations of Amyloid Beta Regulate Recycling of Synaptic Vesicles via Alpha7 Acetylcholine Receptor and CDK5/Calcineurin Signaling

doi: 10.3389/fnmol.2017.00221

Figure Lengend Snippet: CDK5 and calcineurin are involved in Aβ-driven presynaptic plasticity. (A) Quantification of depolarization-induced syt1 Ab uptake from cortical neuronal cultures treated with control, Th and/or roscovitine (Rosc) to evaluate the impact of CDK5. (B) Statistical analysis of depolarization-induced syt1 Ab uptake from cortical cells incubated with vehicle, Th and/or FK506 to investigate the relevance of calcineurin signaling. Numbers within columns in (A,B) represent the number of analyzed cells obtained from three independent cell culture preparations. (C) CDK5 activity assay performed from cortical cultures under different conditions. (D) Representative Western blot as well as quantification of the total CDK5 protein levels from vehicle- or Th-treated cultures. Molecular weight is indicated in kDa. The numbers represent the number of samples from two independent primary culture preparations. (E) Calcineurin activity assay conducted from cortical cells incubated with vehicle or Th. Numbers within columns in (C,E) denote the number of independently treated and analyzed wells in a multi-well dishes obtained from three (C) or four (E) different cell culture preparations. In all graphs, values are expressed as the mean ± SEM. Statistical significance was assessed by one-way ANOVA with Bonferroni post hoc test (A–C) or Student (D) and Mann Whitney t test (E) ; ** p < 0.01, *** p < 0.001.

Article Snippet: Calcineurin activity was assessed using calcineurin cellular activity assay kit (Calbiochem, Cat. No. 207007) according to the manufacturer’s instructions.

Techniques: Control, Incubation, Cell Culture, Activity Assay, Western Blot, Molecular Weight, MANN-WHITNEY

Journal: Molecular medicine reports

Article Title: A preliminary study of the effect of ECRG4 overexpression on the proliferation and apoptosis of human laryngeal cancer cells and the underlying mechanisms.

doi: 10.3892/mmr.2015.4059

Figure Lengend Snippet: Figure 4. Overexpression of ECRG4 induced apoptosis in laryngeal cancer cells through regulation of the expression of apoptosis‑related factors. (A) Examination of apoptosis by flow cytometric analysis. The figure shows the representative results of several repeated experiments. (B) Examination of apoptosis by Hoechst staining. Apoptotic cells exhibited intensive chromatin condensation and dense, strongly Hoechst stained nuclei (magnification, x400). (C) Examination of the expression of Bax, Bcl‑2, cleaved‑caspase‑3 and cleaved‑PARP by western blot analysis. Grayscale analysis was performed using β‑actin as the internal control. Experimental data are expressed as the mean ± standard deviation. Compared with the pcDNA3.1 group, **P<0.01. ECRG4, human esophageal cancer-related gene 4; Bax, Bcl‑2‑associated X protein; Bcl-2, anti‑B‑cell lymphoma 2; PARP, poly ADP‑ribose polymerase.

Article Snippet: The membranes were incubated first with the following primary antibodies: Rabbit anti-human anti-ECRG4 polyclonal antibody (1:200; cat. no. sc-135139; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), rabbit anti-human anti-cleaved-caspase-3 polyclonal antibody (1:500; cat. no. bs-0081R; Bioss, Beijing, China), rabbit anti-human anti-cleaved-poly ADP-ribose polymerase (PARP) polyclonal antibody (1:200; cat. no. sc-23461-R; Santa Cruz Biotechnology, Inc.), rabbit anti-human anti-Bcl-2-associated X protein (Bax) polyclonal antibody (1:400; cat. no. BA0315; Boster, Wuhan, China) and rabbit anti-human anti-B-cell lymphoma 2 (Bcl-2) polyclonal antibody (1:400; cat. no. BA0412; Boster) at 4 ̊C overnight.

Techniques: Over Expression, Expressing, Staining, Western Blot, Control, Standard Deviation

Journal: Cell

Article Title: Vaccine protection against the SARS-CoV-2 Omicron variant in macaques

doi: 10.1016/j.cell.2022.03.024

Figure Lengend Snippet:

Article Snippet: SARS-CoV-2 (Omicron) RBD , Sino Biological , Cat # 40592-V08H121.

Techniques: Infection, Recombinant, Binding Assay, Blocking Assay, Software